ABSTRACT
A dose of 5.0x106 Cryptosporidium parvum oocysts was inoculated in a newborn calf. After the inoculation, the feces were daily collected and the presence of oocysts was examined on slides using 0.17% green malachite dye. The total yield reached 1.5x1010 oocysts, with a peak production on the 7th day, confirming the infectious process and the role of calf infection in the potential risk for environmental contamination.
Subject(s)
Animals , Cryptosporidiosis/chemically induced , Cryptosporidiosis/parasitology , Oocysts , Cattle , Cryptosporidium parvum/isolation & purificationABSTRACT
Disintegration of influenza virions with 0.2% w/v sodium deoxycholate releases haemagglutinin and neuraminidase, which in the presence of detergent are both adsorbed to the lectin from Crotalaria juncea, coupled to Sepharose 2B. The binding is mediated through galactosyl residues on haemagglutinin and neuralminidase, which are completely displaced in 0.2 Μ lactose and co-elute in a narrow zone. The immunogenicity of haemagglutinin and neuraminidase in mice is markedly increased after adsorption onto lipid, particles constituting “intralipid” (Kabi Vitrum, Stocholm, Sweden).